|Contributions||Brunel University. Department of Biochemistry.|
|The Physical Object|
|Number of Pages||310|
Sertoli cells are the primary cellular target for a number of pharmaceutical and environmental testicular toxicants, including 2,5-hexanedione, carbendazim, and mono-(2-ethylhexyl) phthalate. Exposure to these individual compounds can result in impaired Sertoli cell function and subsequent germ cell by: Human Sertoli cells cultured in vitro provide a robust model to monitor environmental toxicant-mediated disruption of Sertoli cell BTB function and to study the mechanism(s) of toxicant-induced. The utility of measuring these products as indices of Sertoli cell function was examined using toxicants targeted to this cell type. Hence, 1,3-dinitrobenzene (1,3-DNB; 10 −6 –10 −4 m), and mono-(2-ethylhexyl)phthalate (MEHP; 10 −8 –10 −4 m) were tested in rat Sertoli cell cultures. Effects of drugs on Leydig cell function BY A. Vermeulen Leydig cell function appears to be much more resistant to the toxic effects of drugs than Sertoli cells and spermatogenesis. The most striking example is probably the azoospermia provoked by cytostatic drugs which leave Leydig cell function and testosterone secretion generally unimpaired.
Exposure to bisphenol A (BPA) in the gestational period damages the reproductive health of offspring; detailed evidence regarding BPA-induced damage in testicular germ cells of offspring is still limited. In this study, pregnant mice (F0) were gavaged with three BPA doses (50 μg, 5 mg, and 50 mg/kg body weight (bw)/day; tolerable daily intake (TDI), no-observed-adverse-effect-level (NOAEL. Bartarseh LI, Welsh MJ and Brabec MJ () Effect of lead acetate on Sertoli cell lactate production and protein synthesis in vitro Cell Biol Toxicol 2: – CrossRef Google Scholar Bellve AR and Feig LA () Cell proliferation in the mammalian testis: biology of seminiferous growth factor. Manganese is an essential trace element that can elicit a variety of toxic responses upon prolonged exposure. The aim of the present study was to elucidate the toxic effects of manganese chloride (MnCl2) exposure on testicular histomorphology, reproductive hormone levels and oxidative stress parameters in Wistar rats. Adult, colony bred male rats were divided into four groups each having eight. This chapter focuses on physical and chemical markers of the testes; stereologic and biochemical assessments of Leydig cells, Sertoli cells, and germ cells; and molecular biologic analyses of DNA and RNA in germ cells. Some markers other than semen analysis (discussed in Chapter 7) are noninvasive or minimally invasive and can be used to assess testicular function in human males exposed to.
Phthalate ester effects on rat Sertoli cell function in vitro: effects of phthalate side chain and age of animal. Heindel JJ(1), Powell CJ. Author information: (1)National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina Primary Sertoli cells (SCs) were treated with NP at various concentrations (0, , 5, 10 or 20 μM) for 12 h. Post-exposure, testicular cells, interstitial tissue and SCs were interrogated respectively using spectrochemical techniques coupled with multivariate analysis. The Sertoli cells catabolize DNB in vitro, creating more polar metabolites through the reactive nitrosonitrobenzene, which produces a similar type of germ cell loss when applied to the cultures and appears more potent than the parent DNB. The hypothesis was put forward that the local production of this metabolite was responsible for the observed toxicity and is one of only three or four . The utility of measuring these products as indices of Sertoli cell function was examined using toxicants targeted to this cell type. Hence, 1,3-dinitrobenzene (1,3-DNB; 10(-6) - 1 The production of lactate and pyruvate as sensitive indices of altered rat Sertoli cell function in vitro following the addition of various testicular toxicants.